Veterinary IVD test
About ASFV:
African swine fever (ASF) is a highly contagious viral disease of domestic and wild pigs, whose mortality rate can reach 100%.
It is not a danger to human health, but it has devastating effects on pig populations and the farming economy. There is currently no effective vaccine against ASF.
The virus is highly resistant in the environment, meaning that it can survive on clothes, boots, wheels, and other materials. It can also survive in various pork products, such as ham, sausages or bacon. Therefore, human behaviours can play an important role in spreading this pig disease across borders if adequate measures are not taken.
African Swine Fever virus (ASFV) antibody test
Do you want to know if a wild pig is infected with the virus? Use the Lomina African Swine Fever Virus test. African swine fever virus (ASFV) is a highly contagious viral disease in domestic pigs and wild pig. (ASFV) is a large double-stranded DNA virus (the only member of the Ascarviridae family) with a complex molecular structure.
Intended Use:
African swine fever virus (ASFV) is a highly contagious viral disease in domestic pigs and wild boars. (ASFV) is a large double-stranded DNA virus (the only member of the Ascarviridae family) with a complex molecular structure. Its large genome, which encodes multiple virulence factors, allows efficient replication, which occurs predominantly in the cytoplasm of monocytes and macrophages. ASFV also has the ability to interfere with cellular signalling pathways, leading to various modulations in the interferon synthesis profiles and other cytokines. ASFV infects domestic pigs, wild boars (Sus scrofa), (also warthogs and bush pigs) as well as soft ticks (Ornithodoros erraticus), which probably act as transmitters. The incubation period is reported to be 4 to 19 days in naturally-acquired cases. The morbidity rate for African swine fever can approach 100% in naïve herds of domesticated pigs. Cumulative mortality depends on the virulence of the isolate, and can range from < 5% to 100%. It is usually 30- 70% in subacute cases.
Video:
* video taken from the “World Organisation for Animal Health” website www.woah.org
Available Packaging Variants:
Usable Biological Material:
The test is developed and tested for wild pigs and for evaluation from a collected blood sample.
Whole blood
Major Parameters, Characteristics & Limits:
P30 strip containing P30 specific antigens labeled by colloidal gold particles.
P54 strip containing P54 specific antigens labeled by colloidal gold particles.
P72 strip containing P72 specific antigens labeled by colloidal gold particles.
The LOMINA ASFV Antibody Test (P30/P54/P72) is a Lateral Flow chromatographic immunoassay for the qualitative detection of P30 antibody, P54 antibody and P72 antibody proteins of the African swine fever virus (ASFV) in veterinary serum or plasma samples in vitro.
P30 protein, P54 protein and P72 protein are expressed in the early, middle and late stage of the ASFV infection. The detection of corresponding antibodies helps in diagnosis and monitoring the complete infection cycle.
Interpretation:
1. Negative test result:
Only clear C line appears.
2. Positive test result:
The presence of both C line and T line (any testing window), regardless of T line being strong or faint.
3. Invalid result:
No colored line appears in C region, regardless of T line’s appearance.
An early stage infection POSITIVE
P30
Developed stage infection POSITIVE
P54
Late stage infection POSITIVE
P72
2 drops of blood and
2 drops of buffer in each window
